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Representative Extracted Ion Chromatograms of Eluforsen and Its Metabolites
In vivo samples were analyzed by LC-MS after sample preparation using a combination of proteinase K digestion and SPE extraction. Mobile phase A consisted of 15 mM DMCHA and 25 mM HFIP in 5% methanol, and mobile phase B containing 95% methanol was used as an optimized buffer system.
