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Validation of hMOR expression in HT1080 cells. (A) Western blot of HT1080 Wild Type (WT) and 3xHA‐OPRM1 lentivirus transduced cells as detected with an anti‐HA monoclonal antibody. (B) Confocal microscopy images detecting amino‐terminal 3x HA‐tagged hMOR using an anti‐HA primary antibody and Alexa488 conjugated secondary. Nucleus is counterstained with Draq5 and shown in red
