Figure 3.

(A) Western blotting of proteins involved in the development of oxaliplatin resistance in SW620 cells. MYC and EHMT2 expression levels increased significantly at high glucose concentrations (15 mM glucose; lane 2), whereas SMAD3 expression level did not. Phosphorylation of SMAD3 and MYC also increased considerably at high glucose concentrations, but, after adding metformin, the increase in phosphorylation could be reversed (lanes 3 and 4). The upregulation of EHMT2 expression could also be reversed after metformin administration (lanes 3 and 4). (B) The proposed mechanism of the effect of high glucose concentrations on the oxaliplatin resistance of CRC.

CRC, Colorectal cancer.