Elevated expression of MTHFD1L in CRC tissues. (A) Gene expression analysis of CRC datasets showed upregulated expression of MTHFD1L in CRC tissues [16]. (B) Dataset from Skrzypczak et al., 2010 [17] showing overexpression of MTHFD1L in CRCs (n = 36) as compared to normal colon tissues (n = 24). (C) TCGA data acquired from UALCAN[18] with MTHFD1L expression in normal colon (n = 41) and CRCs tissues (n = 286). (D) qRT-PCR analysis showing mRNA levels of MTHFD1L in CRCs (n = 110) and matched adjacent non-cancerous tissue (n = 110). (E) Stage-wise expression of MTHFD1L transcripts from UALCAN in CRC tissues Stage I (n = 45), Stage II (n = 110), Stage (n = 80), and Stage IV (n = 39) and normal (n = 41). (F) MTHFD1L mRNA expression in paired samples from different stages of CRC [(Stage 1 (n = 18), Stage 2 (n = 30), Stage 3 (n = 42), and Stage 4 (n = 20)].