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. 2019 May 16;33(9):9797–9810. doi: 10.1096/fj.201802489RR

Figure 4.

Figure 4

Upstream role of p22Phox and p47Phox NOX subunits in promoting TGF-β1 noncanonical signaling. Serum-starved confluent control shRNA and p22Phox shRNA (A) or p47Phox shRNA (E) stably transduced HK-2 cells were treated with TGF-β1. Cellular extracts were immunoblotted with antibodies to p-p53Ser15, pEGFRY845, p-cSrcY416, and GAPDH following confirmation of p22Phox and p47Phox depletion compared to their respective vector control cells (A, E). Histograms in B–D and F–H represent the relative expression (mean ± sd) of indicated phospho-proteins for 3 separate studies. Con, control. *P < 0.05, **P < 0.01.