Figure 5.

The effect of the A23187-induced [Ca²⁺]_i uptake on the spatiotemporal cell behavior in hPDL fibroblasts. A) Pseudocolor images of the cells after stimulation with 20 μM of A23187. B) Representative plot for the numbered cells in A. The arrow indicates the initiation of A23187 treatment. C) Consecutive images of the A23187-induced [Ca²⁺]_i uptake in hPDL fibroblasts taken at 0, 21, 60, 84, 303, 378, 504, and 600 s. The arrowheads indicate the [Ca²⁺]_i response. D) The [Ca²⁺]_i profile of all investigated hPDL cells under A23187 treatment. The average profile is indicated by the block line in black. E) The heatmap and cluster analysis of all the [Ca²⁺]_i signal features. F) The [Ca²⁺]_i signal features and their Moran’s I. ns, not significant. ^‡paired Student’s t test, ^#Wilcoxon signed-rank test, ^{‡, #}P < 0.05, ^‡‡P < 0.01, ^‡‡‡P < 0.001, ^‡‡‡‡P < 0.0001.