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. 2019 Jun 3;33(9):9945–9958. doi: 10.1096/fj.201900338R

Figure 4.

Figure 4

The novel CTS of PC1 interacts with PP1. A) Schematic representation of different GST fusion proteins used in this study. B) Endogenous PP1 in IMCD3 cell lysates pulled down by different GST fusion proteins in A. The Ponceau S–stained GST fusion proteins were used as loading controls. Anti–pan-PP1 antibody detects all isoforms of PP1 (indicated with arrow). IB, immunoblot; N.S, nonspecific band. Below the Western blot images is the quantification result of normalized endogenous PP1 density from ≥3 independent pulldown assays. ***P < 0.001 compared with GST-ΔN44ΔC112 (GST-42). C–E) The GST and GST fusion protein beads were used to pull down HA-PP1α (C), -β (D), and -γ1 (E), which are exogenously expressed in HEK293 cells. Exogenous PP1 proteins were blotted with an antibody against HA. The input and pulldown lanes are from a single gel but were cropped because of discontinuous loading. Quantification results of normalized density of different PP1 isoforms from 3 independent pulldown assays are shown to the right of respective Western blot images. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared with GST-CPC1-PP1 (GST-8).