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. 2019 Jun 3;33(9):9945–9958. doi: 10.1096/fj.201900338R

Figure 6.

Figure 6

PP1α regulates cilium length and ciliation rate. A) quantitative RT-PCR analysis of PP1α depletion by lentiviral short hairpin RNA (shRNA) in IMCD3 cells. The relative knockdown (KD) efficiency of Ppp1ca was around 90%, whereas the expression of Ppp1cb and Ppp1cc was not affected compared with scrambled control. ****P < 0.0001 compared with the scrambled group. B) Western blot analysis confirms the KD of PP1α in IMCD3 cells. C) Depletion of PP1α in IMCD3 cells affected cilium length as evaluated by bisBenzimide H 33342 trihydrochloride (H33342) and acetylated α-tubulin (Ac-α-tub; red) staining. Scale bar, 10 μm. D) The length of cilia in scrambled and PP1α-KD IMCD3 cells at 36 h post serum starvation was measured (n > 1000). Error bars represent sd. ****P < 0.0001 compared with scrambled control. E) Quantification of the percentage of ciliated cells in control and PP1α-KD IMCD3 cells. Error bars represent the sd between microscope fields from ≥3 independent experiments. IB, immunoblot. **P < 0.01 compared with scrambled control.