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. 2019 Jun 3;33(9):9945–9958. doi: 10.1096/fj.201900338R

Figure 7.

Figure 7

PP1α regulates PC1 ciliary localization. A) Depletion of PP1α affected PC1 trafficking to cilia in IMCD3 cells. YFPPC1-HA was visualized with an antibody against GFP (green). Cilia were marked with acetylated α-tubulin (Ac-α-tub; red). Scale bar, 10 μm. B) Percentage of YFPPC1-HA–positive cilia in A. A total of ≥50 ciliated, PC1-transfected cells were counted under each condition. Error bars represent the sd between microscope fields from ≥3 independent experiments. ****P < 0.0001 compared with scrambled control. C) PP1α–knockdown (KD) cells were transiently transfected with YFPPC1-HA alone or together with HA-PP1α. Ciliary expression of PC1 was evaluated by staining with antibody against GFP (green), and cilia were marked with acetylated α-tubulin (Ac-α-tub; red). Representative images of each construct to function are shown. Scale bar, 5 μm. D) Quantification of percentage of YFPPC1-HA–positive cilia in cells. *P < 0.05 compared with the YFPPC1-HA single-transfection group.