TABLE 1.
Physical parameters of IRE1α clusters
| IRE1GFP | Treatment | Sizea | Intensityb | Stability |
|---|---|---|---|---|
| WT | Tm | 0.42 ± 0.02 | 41,771 ± 323 | Transient |
| WT | SubAB | 2.47 ± 3.24 | 465,096 ± 4968 | Persistent |
| WT | 4μ8c | 1.88 ± 0.33 | 365,858 ± 5789 | Persistent |
| K907A | Tm | 1.84 ± 0.32 | 556,822 ± 5514 | Persistent |
| I642G | Tm | 0.32 ± 0.01 | 10,436 ± 21 | Persistent |
| I642G | 1NM-PP1 | 0.37 ± 0.01 | 15,398 ± 42 | Persistent |
| I642G | 1NM-PP1+ Tm | 0.29 ± 0.02 | 7809 ± 15 | Persistent |
a
Cluster size (diameter) measured at peak time and given in micrometers.
b
Integrated cluster fluorescent intensity in arbitrary units.