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. 2019 Oct;130:104496. doi: 10.1016/j.nbd.2019.104496

Fig. 1.

Fig. 1

An ex-vivo system confirms decreased rate of axon degeneration in Wlds and neonatal mice. (A) Representative confocal micrographs of NMJs from age-matched adult C57BL/6 J wildtype and WldS mouse lumbrical muscles after 24 h incubation ex-vivo at 28 °C in oxygenated ringer solution. Note the retention of NMJ integrity in the WldS model compared to the marked loss of pre-synaptic inputs in the wildtype muscle. Scale bar = 25 μm (B) Bar chart (Mean ± SEM) showing the percentage of fully occupied endplates 24 h post-axotomy reveals an increased in the percentage of fully occupied endplates in WldS mice compared to wildtype C57BL/6 J mice. (*p < 0.05 by Mann-Whitney U test; n = 3 preparations per group, data are Mean ± SEM). (C) Representative confocal micrographs of NMJs from the deep lumbrical muscles from P10, P15, P21 and P26 mice after 24 h incubation ex-vivo at 28 °C in oxygenated mammalian physiological saline. Note the increase in vacant endplates (denoted by arrowhead) at P21 and P26 compared to P12 and P15. Scale bar = 25 μm (D) Bar chart (Mean ± SEM) of the percentage of fully occupied endplates in the deep lumbrical muscles from P12, P15, P21 and P26 mice confirms an age-associated decrease in the percentage of fully occupied endplates. (***p < 0.001, by Kruskall-Wallis test; n = 3 preparations per group.