Fig. 4.

Candidate validation of proteomic data by quantitative fluorescent Western blotting. (A) Representative western blots for NAD-dependent deacetylase sirtuin-2 (Sirt2) in nerve and insulin-like growth factor 2 (Igf2) in muscle. All candidate blots were normalized to total protein load (Ponceau S stain) (B,C) Scatter plots (individual data points; Mean ± SEM) of relative expression of Sirt2 (B) and Igf2 (C) relative to P12. Note direction and magnitude of change is consistent with TMT data. (n = 3 pairs of nerves (SIRT2) or 8 lumbrical muscles (IGF2) per time point; **p < 0.01, ***P < 0.001 by Kruskall-Wallis test).