Fig. 5.

Combined analysis in nerve and muscle reveals conserved escalation in mitochondrial oxidative phosphorylation related proteins. Use of the Path Designer function in IPA software, to reveal combined interaction network, reveals an overlap in molecular interactions pertaining to the mitochondrial oxidative phosphorylation process. Specifically, candidates identified as components of mitochondrial Complex I (NADH dehydrogenase) and Complex IV (cytochrome c oxidase) exhibit conserved expression changes in both nerve and muscle data sets. Proteins within this network reflect those which up-regulated (red) and down-regulated (green) during the P12-P24 time-course in either nerve or muscle; intensity of colour corresponds with magnitude of expression change. White represents a molecule that is absent from both datasets but an important component of the network. Grey represents a molecule that is present in the datasets but the change in levels is below the 20% cut-off. Solid connecting lines represent a direct interaction, while dashed connecting lines indicate an indirect interaction. Interactions conserved between nerve and muscle data sets are highlighted as purple connecting lines. Blue connecting lines represent the highlighted interactions of NADH dehydrogenase. All suggested indirect interactions were confirmed manually using the IPA software to identify publications indicating an experimentally reported interaction between the two components. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)