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. 2019 Oct;130:104496. doi: 10.1016/j.nbd.2019.104496

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© 2019 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fig. 7 — Rotenone protects axons and ameliorates rise in ROS in a DRG model of axon injury. A) Fluorescent images show axons from dorsal root ganglion primary cultures labelled with antibodies against neurofilament heavy chain (NF-H) which are either non-injured or 12 h post axotomy. Note widespread axon fragmentation following axotomy which is prevented by treatment with 10 μM rotenone. Scale bar = 20 μM B) Bar chart (Mean ± SEM) shown axon degeneration index in non-injured (Control/Rot) on injured (Axot/Axot + Rot) cultures which were either untreated (Control/Axot) or exposed to 10 μM Rotenone (Rot/Axot+Rot). **** P < 0.0001 Axot compared to all other groups, ANOVA with Tukeys multiple comparison test. C) Fluorescent images show axons from dorsal root ganglion primary cultures labelled with DHE which are either non-injured or 6 h post axotomy. Note increase in fluorescence following axotomy which is not observed following treatment with 10 μM rotenone. Scale bar = 20 μM D Bar chart (Mean ± SEM) shown DHE fluorescence in non-injured (Control/Rot) on injured (Axot/Axot + Rot) cultures which were either untreated (Control/Axot) or exposed to 10 μM Rotenone (Rot/Axot + Rot). **P < 0.01; *P < 0.05; ANOVA with Tukeys multiple comparison test.