Skip to main content
. Author manuscript; available in PMC: 2019 Aug 22.
Published in final edited form as: Methods Mol Biol. 2019;1877:77–91. doi: 10.1007/978-1-4939-8861-7_5

Fig. 4.

Fig. 4

Representative examples of intracellular flow for (a) BCL-XL and (b) BIM in human diffuse large B cell lymphoma cell lines Pfeiffer and OCI-Ly8. The positive gate is drawn using the IgG isotype control (left column). This gate is copied over to the samples incubated with either BCL-XL-PE or BIM-PE antibodies (center column). An overlay of the samples with their isotype controls is shown in the right column. Western blotting of each cell line reveals the relative protein levels of BCL-XL and BIM. As reflected in protein lysate and intracellular flow, Pfeiffer has higher relative amounts of BCL-XL and BIM compared to OCI-Ly8