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. Author manuscript; available in PMC: 2019 Aug 22.
Published in final edited form as: J Hepatol. 2018 Oct 25;70(3):458–469. doi: 10.1016/j.jhep.2018.10.015

Fig. 7. Loss of LSEC autophagy is associated with an insufficient antioxidant response.

Fig. 7.

Atg7endo and Atg7control mice were treated every other day with CCl4 i. p. for 1 week to induce mild acute liver injury and primary LSECs were isolated. (A) mRNA changes (qPCR analysis) showing a downregulation of the classical genes that protect against oxidative stress Gpx1, Gpx4, Sod1, Sod2 and Cat and (B) mRNA changes (qPCR analysis) showing upregulation of the Nrf2-dependent antioxidative stress genes Srxn, Nqol, Gclc, Gclm, and Gstm2. (C) Immunoblots for NQO1 and HMOX1 in isolated LSEC from Atg7endo and Atg7control mice confirming an increase at the protein levels. Data shows mean value ± SEM of at least 3 experiments. mRNA expression is expressed as fold-change relative to control (*p ≤0.05, **p ≤0.01, ***p ≤0.001, Student’s t test). LSEC, liver sinusoid endothelial cell; qPCR, quantitative real-time PCR