Figure 4.

Inhibition of SP1 may mimic the inhibitory effects of miR-548b overexpression in HCC cells. (A) Huh7 and Hep3B cells that were transfected with either SP1 siRNA or NC siRNA, respectively, were subjected to western blot analysis for SP1 protein expression. (B) Cell Counting Kit-8 assay was performed to assess the cellular proliferation in Huh7 and Hep3B cells following SP1 knockdown. (C) Invasive capacity was tested using invasion assays after SP1 knockdown in Huh7 and Hep3B cells. *P<0.05 vs. NC siRNA. miR, microRNA; SP1, specificity protein 1; HCC, hepatocellular carcinoma; NC, negative control; si, small interference.