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. 2019 Jul 25;18(3):2332–2340. doi: 10.3892/etm.2019.7812

Figure 5.

Figure 5.

miR-548b exerts its tumor-suppressing effects on HCC cell proliferation and invasion by suppressing SP1 expression. (A) The expression of SP1 mRNA in Huh7 and Hep3B cells after pcDNA3.1-SP1 (pc-SP1) or empty pcDNA3.1 plasmid transfection was analyzed via RT-qPCR analysis. *P<0.05 vs. pcDNA3.1. (B) Western blot analysis was carried out to measure SP1 protein expression in miR-548b-overexpressing Huh7 and Hep3B cells that were transfected with either pc-SP1 or pcDNA3.1. (C) Cell Counting Kit-8 and (D) invasion assays were performed to determine the proliferation and invasion of Huh7 and Hep3B cells, respectively following co-transfection with miR-548b mimics, and either pc-SP1 or pcDNA3.1. *P<0.05 vs. miR-NC; #P<0.05 vs. miR-548b mimics+pcDNA3.1.