FIG 6.

Effect of deletions of four of the five effectors, sifA, spvB, sseF, sseJ, and steA, on T3SS-1-independent inflammation in mice. (A) LDH release from RAW cells infected with the indicated S. Typhimurium mutant strains at 20 h after infection. (B) Transcript levels of Ifng, Il6, Tnfa, Kc, and Mip2 in the ceca of mice infected with the indicated S. Typhimurium strains at 5 days after infection, as measured by qPCR. (C) Combined histopathological scoring of cecal sections. A two-tailed Student t test (A) and the Mann-Whitney U test (B and C) were performed for statistical analysis. Individual data are shown as a scatter plot, and bars indicate the means (A and B). Each plot represents an individual mouse (C). Asterisks indicate differences that were statistically significant (P < 0.05). NS, not significant.