FIG 1.

Adherence and viability of A549 lung epithelial cells following infection with S. maltophilia wild-type, virB10 mutant, and virB10 xpsF mutant strains. (A and B) A549 cell monolayers were either uninfected (UI) or infected with parental strain K279a (WT), virB10 mutant NUS15 (virB10), or complemented mutant NUS15(pvirB10) (virB10/virB10+). At 8 h postinoculation, the amounts of adherent host cells were enumerated (A), and at 24 h postinoculation, the total numbers of viable A549 cells (both attached and detached) were determined (B). (C) A549 cells were uninfected or infected with either the virB10 xpsF double mutant NUS16 (virB10 xpsF) or one of the three other strains indicated in panels A and B, and at 24 h postinoculation, the numbers of viable host cells (both attached and detached) were determined. For panels A to C, the raw data appear in the left panels, and data normalized to the value for uninfected cells are in the right panels. Asterisks indicate significant differences in adherence or viability (*, P < 0.05; **, P < 0.01). Data are presented as the means and standard deviations of results from three independent experiments (n = 3 each).