FIG 8.

Bactericidal effect of S. maltophilia wild-type and virB10 mutant strains on various species of Pseudomonas. S. maltophilia K279a (WT), virB10 mutant NUS15 (virB10), or complemented mutant NUS15(pvirB10) (virB10/virB10+) was mixed with P. mendocina (A), P. fluorescens (B), P. putida (C), and P. stutzeri (D) at a ratio of ∼100:1 and spotted onto LB agar, and after 2 h of incubation, the numbers of each strain were determined by plating on selective media. The results presented are the CFU at t = 0 h and t = 2 h for each of the P. aeruginosa strains that had been coincubated with the WT, mutant, and complemented mutant S. maltophilia (left) as well as the CFU for the S. maltophilia strains (right). Asterisks indicate significant differences in CFU obtained at t = 0 versus t = 2 h (*, P < 0.05). Data are presented as the means and standard deviations of results from three independent experiments (n = 3 each).