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. 2019 Aug 21;87(9):e00243-19. doi: 10.1128/IAI.00243-19

FIG 4.

FIG 4

Effects of A. baumannii OMVs on cytokine release by macrophages and regulation by TLR2 and TLR4. (A and B) Various concentrations of A. baumannii OMVs (0, 0.01, 0.1, and 1 ng/ml in total protein concentrations) were added to RAW264.7 cells (mouse macrophage cell line) in vitro, and at 12 h after stimulation with the OMVs, the concentrations of IL-6 (A) and TNF-α (B) in conditioned media were measured. (C) Peritoneal macrophages from wild-type, MyD88 knockout (KO), TLR2 KO, and TLR4 KO mice were stimulated with various concentrations of A. baumannii OMVs (0, 0.01, 0.1, and 1 ng/ml in total protein concentrations). At 12 h after stimulation with the OMVs, the concentrations of IL-6 in conditioned media were measured. *, P < 0.05; ***, P < 0.001.