FIG 8.

A. baumannii OMVs activate interferon gamma in the lung. To understand if OMV contributes to the activation of the interferon pathway, we measured expression of interferon genes in the samples mentioned in Fig. 7 using qPCR. The levels of interferon alpha mRNA (A) and interferon beta mRNA (B) were not different in OMV-treated mice from those in the controls. In contrast, expression of interferon gamma was significantly elevated in the lungs of mice that were intranasally administered with OMVs (C), suggesting specificity of interferon activation by OMVs. ns, not significant; ***, P < 0.005. n = 6 mice in each group.