TABLE 2.
Plasmidsa
| Plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| pTVm2 | NorV-His expression plasmid; Ampr; Ptrc | 10 |
| pThmp1 | Hmp-His expression plasmid; Ampr; Ptrc | This study |
| pBhcp1 | Hcp expression plasmid; Cmr; PBAD | This study |
| pBnorR2 | NorR expression plasmid; Cmr; PBAD | This study |
| pBnsrR2 | NsrR expression plasmid; Cmr; PBAD | This study |
| pBfnr1 | Fnr expression plasmid; Cmr; PBAD | This study |
| pTrcHis 2C | Expression plasmid; Ampr; Ptrc | Invitrogen |
| pBAD33 | Expression plasmid; p15-ori PBAD; Cmr | National BioResource Project (NIG) (Japan) |
| pRPL3 | NO reporter plasmid; Ampr | 10 |
| phmpC10 | Suicide plasmid; hmp and loxP-flanked Cmr cassette | This study |
| phmp100C2 | Suicide plasmid; deleted hmp(1–300) and loxP-flanked Cmr cassette | This study |
| phmp305C15 | Suicide plasmid; deleted hmp(1–915) and loxP-flanked Cmr cassette | This study |
| phmpCF43A1 | Suicide plasmid; mutated hmp(F43A) and loxP-flanked Cmr cassette | This study |
| phmpCF390A1 | Suicide plasmid; mutated hmp(F390A) and loxP-flanked Cmr cassette | This study |
| pRed/ET(amp) | Red/ET expression plasmid; Ampr | Gene Bridges GmbH |
| pFRT-Kan | Suicide plasmid; FRT-flanked PGK-gb2-neo cassette | Gene Bridges GmbH |
| ploxCm1 | Suicide plasmid; loxP-flanked Cmr cassette | 41 |
| ploxSm11 | Suicide plasmid; loxP-flanked Smr cassette | This study |
| pAH144 | Source of Smr cassette | National BioResource Project |
| pCreA1 | Thermosensitive Cre expression plasmid; Ampr | 41 |
| pFT-A | Thermosensitive FLP expression plasmid; Ampr | National BioResource Project |
| pLCE19 | Suicide plasmid; luxE and loxP-flanked Cmr cassette | 41 |
| pluxCDAB3 | LuxCDAB expression plasmid; Ampr; Plac | 41 |
a
Ampr, ampicillin resistant; Cmr, chloramphenicol resistant; Smr, streptomycin resistant.