Fig. 1.
Effects of topoisomerase inhibitors on cell motility in vitro and in vivo. a, b VP-16, ADM and CPT-11 promote the migration (a) and invasion (b) of LoVo, SW480 and H446 cells. Cells were subjected to transwell chamber assays for 24 h in the presence of indicated concentrations of agents. c VP-16 and CPT-11 promote the metastasis in vivo. After treatment with VP-16 (20 μM) or CPT-11 (80 μg/ml) for 0.5 h, LoVo cells (5 × 105 per animal) were injected to the caudal vein of NOD/SCID mice (n = 8 per group). After 56 days, lungs were stripped for counting nodules. Upper, macroscopic observation of lungs of mice. Lower, graph summarizing the number of metastasis nodules. d Hematoxylin-eosin staining of the lung tissues of mice