Fig. 4.

The level of T cell-mediated damage on neurons was significantly reduced following in vitro treatment of co-cultured T cells and neurons with an anti-TIM-1 antibody. a-b Representative images and quantification of an Edu incorporation assay for cellular proliferation. **p < 0.01. c LDH expression from T cell + neuron cells in each of the different treatment groups. **p < 0.01. d Light microscopy for detecting neuronal cell morphology. e-h An ELISA was used to examine the changes in cytokine secretion, including TNF-α, MIP-1α, NO, and IL-6 in each group. *p < 0.05; **p < 0.01