Fig. 2.

Bacterial interactions regulating P-gp inhibition. a Effects of GBS infection on R123 accumulation in an MDCK cell line that overexpresses human MDR1. Multiplicity of infection (MOI) was varied and after 5 h of infection, P-gp function was compared to uninfected cells and CsA treated cells. b Effects of live GBS, GBS-conditioned medium (CM) and heat-treated non-viable (Heat) or paraformaldehyde-fixed (PFA) GBS on R123 accumulation in iPSC-derived BECs after 5 h of treatment. c iPSC-derived BECs were infected with GBS mutants or wild-type (WT) (MOI = 10) for 5 h, and R123 accumulation compared to uninfected controls. Experiments were performed in triplicate on three independent biological replicates (a) or differentiations (b, c). All raw data are presented (n = 9), and are expressed as % of uninfected control BEC accumulation. All experiments and bars represent mean ± SD. **p < 0.01, ***p < 0.001, NS p > 0.05, versus uninfected control unless otherwise specified; ANOVA followed by Dunnett’s multiple comparisons test