Fig. 4.

SIX1 promotes the progression of gastric cancer by transcriptionally regulating Vimentin expression. a The level of SIX1 positively correlated with the level of Vimentin in 61 paired gastric cancer tissues by Pearson correlation analysis (p < 0.01). b and c Relative expression of SIX1 and Vimentin was detected by qRT-PCR and western blotting in gastric cancer cells after transfection of LV-shSIX1, pLVX-SIX1 or negative control. d Schematic illustration of the sequences of wild type of Vimentin promoter domain and mutant sequences in the binding sites of SIX1 on Vimentin promoter domain are shown. e and f Relative luciferase activities were detected in gastric cancer cells after transfecting luciferase reporter plasmids with wild type of Vimentin promoter domain or mutant Vimentin promoter domain with LV-shSIX1, pLVX-SIX1 or negative control. g-j The effects of SIX1 on the mobility, migration and invasion were detected by wound healing and transwell assays. All data are presented as the mean ± SEM of three experiments. **p < 0.01