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. 2019 Aug 22;19:829. doi: 10.1186/s12885-019-6015-4

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Expression of MMP-2 and MMP-9 in A549 and H1299 cells treated with ¹²C with and without olaparib (O). a Relative mRNA expression of MMP-2 and MMP-9 in A549 cells after 24 h treatment as determined by Real Time PCR. Each bar represents mean expression ± standard deviation obtained from three independent experiments. All the differences with respect to control are significant and p-values at each dose was p ≤ 0.001 in each cell-type. b-c & d-e Typical western blot to determine expression of MMP-2 and MMP-9 from whole cell lysate in b (A549) & d (H1299) and their respective secretion in culture medium in c (A549) & e (H1299). f,g, h & i Relative expression as measured in fold of MMP-2 and MMP-9 from whole cell lysate f (A549) & h (H1299) and their respective secretion in culture medium in g (A549) & i (H1299)