Figure 4.
Involvement of TRPV1 channels in IL-1β-mediated suppression of striatal CB1Rs(GABA). A, B, IL-1β, applied intracerebroventricularly (A, n = 6) or in the slice bathing solution (B, n = 6), failed to block the sensitivity of CB1Rs(GABA) to HU210 in mice lacking TRPV1 channels. C, D, SDS failed to block the sensitivity of CB1Rs(GABA) to HU210 in mice lacking TRPV1 channels (C, n = 5) or in WT mice treated with a single intraperitoneal injection of I-RTX, a blocker of TRPV1 channels (D, n = 6). E, F, Histograms show that the binding of TRPV1 agonist [3H]RTX (RTX*) was not affected by the presence of IL-1β either in the striatum (E, n = 3) or in the spinal cord (F, n = 3). Unspecific binding was determined in the presence of the cold agonist RTX or of the selective antagonist I-RTX. *p < 0.05.