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. 2012 Oct 24;32(43):15012–15026. doi: 10.1523/JNEUROSCI.2248-12.2012

Figure 2.

Figure 2.

mTOR signaling occurs in TA progenitor cells but not GFAP+ cells within the adult forebrain SVZ niche. A, Illustration of the principal cell types in the SVZ neurogenic lineage and their associated cell type-specific markers. B, Immunostaining for P-S6-RP (red) reveals expression in only a discrete subset of SVZ cells. Nuclei are counterstained with Hoechst. C–H, Multilabel immunofluorescence for P-S6-RP (green) and cell type-specific markers (red). The vast majority of P-S6-RP-positive cells are proliferating TA progenitors as defined by expression of Ki67 (E), Mash1 (F) and/or Olig2 (H). I, J, Quantification of immunofluorescence experiments, expressed as a percentage of P-S6-RP cells (I) or as a percentage of marker-positive cells (J). K, Immunofluorescence staining for Mash1 (green) and P-4EBP1 (red) in the SVZ. L, Immunofluorescence staining for GFP (green) and P-S6-RP (red) in the SVZ of GFAP-GFP transgenic mice. Scale bars: (in H) C–H, 25 μm; K, L, 40 μm. Arrows indicate double-labelled cells.