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. 2013 Jan 9;33(2):734–747. doi: 10.1523/JNEUROSCI.4390-12.2013

Figure 2.

Figure 2.

Primary afferent VGluT1-immunoreactive innervation of VF neurons. A, B, The confocal micrograph in A demonstrates specific immunostaining for VGluT1 (yellow) in a cross section through the S2 segment, which is completely eliminated by pretreatment with the blocking peptide of the primary VGluT1 antibodies (B). C, Low-power projected confocal micrographs of a 50-μm cross section cut through the S2 segment of the spinal cord shows VF neurons retrogradely labeled through the left VF at the S1–S2 level (red) and anterogradely filled sacral afferents (cyan) labeled via the contralateral S3 dorsal root. D, The labeled VF neurons and afferents displayed in C are shown after VGluT1 immunostaining (yellow). E, F, High-power (original magnification × 60), single optical slice micrographs of the VF neurons in the area framed in D are shown in E and F. E, VF neurons and afferents. F, VF neurons contacted by primary afferent boutons IR for VGluT1 (yellow). Fluorophores: VF, cascade blue dextran (pseudo-color, red); afferents, Texas red dextran (pseudo-color, cyan); and VGluT1, Cy5.