Figure 12.

Structural alteration of the Golgi apparatus in neurons lacking RIM3γ/4γ. A, Confocal images of GM130-labeled cultured hippocampal neurons, transfected at DIV 3 with either shRNAs against RIM3γ (shRIM3) or RIM4γ (shRIM4), or mutated variants of the both shRNAs (mutated shRIM3, mutated shRIM4), or GFP alone (control). Cells were fixed at DIV 14 and stained against the Golgi marker GM130 (red). Scale bar, 50 μm; * 20 μm. B, Quantification of Golgi dispersion. While Golgi dispersion in RIM4γ knock-down cells is indistinguishable from control, RIM3γ knock-down leads to increased fragmentation and dispersion. C, Quantification of Golgi size shows that knock-down of RIM4γ leads to a smaller, more condensed Golgi apparatus as compared with controls. B, C, These structural alterations were abolished using the mutated shRNAs against RIM3 or RIM4. Significance: one-way ANOVA followed by Tukey's multiple-comparison test, ***p < 0.0001.