RIM3γ and RIM4γ knock-down decreases miniature excitatory synaptic activity. A, Representative recordings of mEPSCs from a control neuron (gray), and neurons with reduced levels of either RIM3γ (RIM3γ shRNA; black) or RIM4γ (RIM4γ shRNA; red). B, C, Analysis of mEPSC frequency and amplitude after RIM3γ or RIM4γ knock-down. B, Both RIM3γ and RIM4γ knock-down cause significant reduction in mEPSC frequency (p < 0.01 and p < 0.05, respectively; one-way ANOVA with Dunnett's post-test). C, RIM3γ knock-down causes a significant decrease of the mean amplitude (p < 0.05; one-way ANOVA with Dunnett's post-test; n = 7, 6, and 5 for RIM3γ and RIM4γ knock-down and control neurons, respectively).