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. 2013 Jan 9;33(2):556–562. doi: 10.1523/JNEUROSCI.4568-12.2013

Figure 1.

Figure 1.

Single-cell expression analysis in PLL neurons. A, The schedule of zebrafish PLL development and our experimental procedure. B, Quantitative representation of initial mRNA abundance of external control genes (on the horizontal axis) after cDNA amplification (on the vertical axis). We examined four control RNAs of ERCC RNA Spike-In Mix (ERCC00035, 00042, 00136, 00130) and three synthesized mRNAs (Lys, Dap, and Phe, derived from Bacillus subtilis). C, The expression pattern of stmn1b at 24 hpf. stmn1b is expressed in the PLL ganglion (arrowhead). D, GFP is expressed in the PLL ganglion (arrowhead) in the Tol047 embryo at 3 dpf. Scale bars, 0.5 mm. Insets show higher magnifications of the PLL ganglion. E, The expression levels of three housekeeping genes (red dots), two PLL neuron markers (green dots), and four neurogenesis markers (blue dots), in single PLL neurons (n = 57). ND, Not detected.