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. 2013 Jan 9;33(2):595–610. doi: 10.1523/JNEUROSCI.2583-12.2013

Figure 2.

Figure 2.

Characterization of an NHE6 isoform-specific rabbit polyclonal antibody by ICC. A, Colocalization of native NHE6 with the Tf-R in the soma and neurites of cultured human SH-SY5Y neuroblastoma cells. Scale bar, 5 μm. B, Control experiment showing that the signal detected with the NHE6 antibody is specific in SH-SY5Y neuroblastoma cells, as no fluorescent signal is observed in the presence of immunizing antigen (ag). Nuclei were stained with DAPI. The green Alexa Fluor 488-conjugated secondary goat anti-rabbit antibody was applied to both samples. Scale bar, 10 μm.