Figure 3.

Subcellular localization of native NHE6 in areas CA3 and CA1 of mouse hippocampus by TEM. A, In control experiments, no signals were observed in hippocampal area CA3 in the absence of primary antibody, demonstrating the specificity of the signal produced by the NHE6 antibody. B, NHE6-positive puncta were present mostly in dendrites (sometimes close to PSDs) of hippocampal neurons in area CA3, but are occasionally associated with presynaptic boutons. C, NHE6 immunogold-positive puncta were detected in dendrites in hippocampal area CA1. Magnification: ×24400. SV, Synaptic vesicles; PSD, postsynaptic density; mito, mitochondria.