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. 2013 Jan 9;33(2):544–555. doi: 10.1523/JNEUROSCI.2255-12.2013

Figure 2.

Figure 2.

Chemical characterization of three distinct subgroups of GABAergic neurons in the S1. A–C″, We performed double immunofluorescence labeling for PV, SOM, and VIP, and found no overlap in the cell bodies between these chemical markers in the mouse S1. D–F″, GAD67 mRNA and the immunoreactivity for PV, SOM, or VIP were visualized with FastRed (magenta) and AF488 (green), respectively. Almost all of the cells immunoreactive for PV, SOM, or VIP displayed the signals for GAD67 mRNA (arrowheads). G–I, PV, SOM, or VIP immunoreactivity in VGAT-immunopositive terminal-like structures in the S1. VGAT and PV, SOM, or VIP immunoreactivities were visualized with AF488 (green) and AF647 (magenta), respectively. We randomly selected six sites in the middle portion of each layer of the S1 in three wild mice, acquired 3D image stacks under a confocal laser-scanning microscope, and the colocalization of the immunoreactivities in the axon terminal-like structures (arrowheads). Approximately 80 of VGAT-positive terminals at each site were analyzed in the present study. Arrows in G indicated the presumed dendrites or axon fibers, which were not punctate but usually showed long or straight profiles in the 3D stack. Scale bars: (in F″) A–F′, 20 μm; (in I) G, H, 2 μm.