Figure 5.
Patch-clamp recordings from postsynaptic SGN boutons of BsnΔEx4/5 mutants. A, B, Synaptic regions of IHCs of p11 WTΔEx4/5 (A) and BsnΔEx4/5 (B) mice, double-stained for GluA2 (blue) and CtBP2 (red), illustrating a relatively high ribbon occupancy in immature mutant IHC. C, Sample recordings of EPSCs from postsynaptic boutons of type I SGNs in WTΔEx4/5(left) and BsnΔEx4/5 (right). D, Frequency of EPSCs at rest (in artificial perilymph containing 5 mm K+) and during IHC stimulation by application of artificial perilymph containing 40 mm K+ for WTΔEx4/5 (filled circles) and BsnΔEx4/5 (open circles). E, Sample traces of EPSCs in a millisecond scale to demonstrate their kinetics. Both monophasic and multiphasic EPSCs from WTΔEx4/5 and BsnΔEx4/5 boutons are shown. F, Percentage of monophasic EPSCs derived from individual afferents of WTΔEx4/5 and BsnΔEx4/5 SGN, respectively.