Figure 5.

Impact of ABCA7 deletion on Aβ phagocytosis and brain macrophage/microglial markers. A, Bone marrow-derived macrophages from WT and Abca7^−/− mice were incubated with 5 μm Aβ oligomers (left) for 45 min. Cells were rinsed and lysates were probed for Aβ uptake by Western blotting. β-Actin was used as a loading control. B, Integrated optical density for the Western blot is shown. Levels are expressed relative to WT mice and are mean ± SE. **p < 0.01 compared with J20. C, Hippocampal sections were stained for Aβ, Iba1, and Mac2. Images were collected using confocal microscopy and merged as indicated. Scale bar, 50 μm. D, Iba1 and Mac2 levels in brain homogenates were assessed by Western blotting. Representative blots (n = 4 samples) are shown. β-Actin was used as a loading control. E, Iba1 and Mac2 in hippocampal sections were assessed by immunohistochemistry. Scale bars: Top, 500 μm; bottom, 50 μm. F, Quantification of Iba1 and Mac2 blots (Western, J20, n = 14; J20/A7^−/−, n = 11) and immunohistochemistry (IHC, J20, n = 6; J20/A7^−/−, n = 6) is shown. Levels are expressed relative to J20 mice and are mean ± SE.