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. 2013 Mar 20;33(12):5127–5137. doi: 10.1523/JNEUROSCI.0600-12.2013

Figure 6.

Figure 6.

Effects of cell-type-selective miRNAs on neural stem cell differentiation. A, Neural stem cell cultures before differentiation labeled with anti-nestin antibody. Scale bar, 15 μm. B, Neural cultures after FGF withdrawal and differentiation labeled with anti-Tuj1 antibody (against Tubb3 protein). Scale bar, 15 μm. C, Overexpression of neuron-enriched miRNAs miR-124, miR-434, and miR-376a via lentivirus-mediated transduction in neural stem cells increases the number of Tuj1+ cells, whereas overexpression of glial-enriched miRNAs miR-223, miR-146a, miR-19a, and miR-32 in neural stem cells decreases the number of Tuj1+ cells. Control samples comprised parallel cultures not exposed to lentiviral vectors. The mean of 15 independent replicates is shown; error bars represent SEM. **p < 0.01 by unpaired t test compared with control.