Figure 4.

The EB and MB structures are close enough to form synaptic connections. A–F, The GRASP signal (green) reconstituted by MB 247-LexA–driven GFP11 and EB c819-GAL4–driven GFP1–10 (A–C) was partially reduced by Gad-GAL80 (D–F). A, D, Projection view of the MB-EB GRASP signal. B1–C2, Single section of the MB-EB GRASP signal revealing putative connections (green) between the MB and EB in the EB RF tract area (B1, B2) and the ring area (C1, C2). E1–F2, Single section of the MB-EB GRASP signal under the suppression of Gad-GAL80 showed weakened signals in both areas. Anti-synapsin counterstaining is shown in red (B2, C2, E2, F2). Scale bar, 50 μm.