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. 2013 Mar 20;33(12):5120–5126. doi: 10.1523/JNEUROSCI.2364-12.2013

Figure 2.

Figure 2.

c-Fos expression in the AOB neurons of sexually naive males and fathers after pup exposure. A–F, Representative microscopic images of c-Fos (black) and Gαo (brown) expression in the AOB of sexually naive males (A, C) and fathers (B, D) in control condition (A, B) and after pup exposure (C, D). E, F, Higher magnification of C and D. Scale bars, 100 μm. G–I, The numbers of c-Fos-positive cells in the glomerular layer (GL) (G), mitral/tufted cell layer (MCL) (H), and granule cell layer (GCL) (I) in the rostral and caudal zones of the AOB in sexually naive males and fathers with or without pup exposure (n = 6). Welch's ANOVA revealed significant differences among groups in both rostral and caudal zones of the GL (rostral, F(3,9.14) = 23.75, p < 0.001; caudal, F(3,9.89) = 7.37, p = 0.007), MCL (rostral, F(3,9.61) = 12.40, p = 0.001; caudal, F(3,10.58) = 4.58, p = 0.027), and GCL (rostral, F(3,10.09) = 7.59, p = 0.006; caudal, F(3,10.69) = 10.68, p = 0.002). Data are represented as mean ± SEM. *p < 0.05, **p < 0.01 (Welch's t test followed by correction with Holm's method). NS, Not significant.