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. 2019 Aug 7;4(8):13015–13026. doi: 10.1021/acsomega.9b00489

Figure 3.

Figure 3

Quantification of multi-LP/Cy5-eGFP-mRNA (a,b) and mono-LP/Cy5-eGFP-mRNA (c,d) uptake by APCs in the spleen (a,c) and ILNs (b,d) 24 h after i.v. or i.d. immunization with 20 μg of Cy5-eGFP-mRNA. In vivo transfection efficiency of APCs in the spleen (e) and ILNs (f) 24 h after i.v. injection of 20 μg of eGFP-mRNA loaded into multi-LP. Values represent the mean ± SD of two separate experiments involving three to five mice per group. Values were considered significant at *p < 0.05, **p < 0.01, ***p < 0.001, n.s. = not significant. Statistical significance was evaluated using a nonparametric one-way ANOVA with a Dunn’s post-test.