Figure 1.

Anatomy and synaptic connectivity of L. stagnalis B1 and B2 neurons. Ai, Micrograph of isolated BGs showing soma position of B1 and B2 neurons (outline marked by dotted lines). Aii, Maximum-intensity Z projection of two-photon laser scanning image of the BGs shown in Ai after intracellular injection of a B1 neuron with Alexa Fluor 568 and a B2 neuron with Alexa Fluor 488. B, Schematic diagram showing the electrical coupling between the left and right B1 neurons and the innervation of the salivary glands by the B1 neurons and the esophagus by the B2 neurons. BBC indicates buccal-buccal commissure; CBC, cerebral-buccal connective.