Table 1.
Electrophysiological properties of B1 and B2 neurons
| Vm (mV) | Input resistance (MΩ) | AP |
|||
|---|---|---|---|---|---|
| Amplitude (mV) | Width (ms) | AHP (mV) | |||
| B1 | |||||
| DIV2+ | −68.7 ± 1.3 (25) | 18.7 ± 2.0 (11) | 75.0 ± 1.5 (24) | 6.1 ± 0.5 (24) | −14.3 ± 0.5 (24) |
| DIV2+/7NI | −67.7 ± 1.2 (26) | 23.2 ± 2.6 (9) | 75.6 ± 1.2 (18) | 7.5 ± 0.5 (18)* | −15.2 ± 0.7 (18) |
| DIV2+/7NI+DETA | −67.6 ± 1.2 (34) | 21.4 ± 2.0 (16) | 72.5 ± 1.1 (22) | 6.5 ± 0.2 (22) | −15.8 ± 0.6 (22) |
| DIV2+/ODQ | −74.8 ± 0.8 (29)* | 25.6 ± 1.4 (12) | 72.0 ± 1.8 (16) | 6.4 ± 0.4 (16) | −15.1 ± 1.4 (16) |
| DIV2+/KT5823 | −64.1 ± 0.9 (30)* | 20.2 ± 1.5 (12) | 71.3 ± 1.2 (26) | 5.2 ± 0.2 (26) | −14.5 ± 0.4 (26) |
| B2 | |||||
| DIV2+ | −61.8 ± 1.1 (24) | 18.7 ± 2.1 (7) | 76.4 ± 1.2 (24) | 9.8 ± 0.7 (24) | −17.0 ± 0.8 (24) |
| DIV2+/7NI | −65.5 ± 1.2 (27) | 22.7 ± 2.4 (12) | 78.0 ± 1.8 (26) | 12.6 ± 1.0 (26) | −16.2 ± 0.7 (26) |
| DIV2+/7NI+DETA | −66.1 ± 1.3 (30) | 24.1 ± 2.0 (15) | 77.7 ± 0.9 (28) | 13.9 ± 0.9 (28)* | −16.3 ± 0.7 (28) |
| DIV2+/ODQ | −72.6 ± 1.4 (24)* | 28.9 ± 1.5 (9)* | 70.1 ± 1.2 (20)* | 9.4 ± 1.0 (20) | −15.6 ± 0.7 (20) |
| DIV2+/KT5823 | −59.8 ± 1.2 (31) | 25.0 ± 2.6 (11) | 73.5 ± 1.0 (29) | 9.5 ± 0.4 (29) | −18.8 ± 0.5 (29) |
Show is the average Vm, input resistance, AP amplitude, AP width, and amplitude of AP AHP of B1 and B2 neurons after DBN crushing and organ culture for 2 d in the different treatment groups. The measurements were conducted in NS. AP width was measured at 50% of the peak amplitude. AHP was measured as the amplitude of the negative peak after the AP relative to the base of the AP. Data are shown as mean ± SEM (n). Statistical analysis was carried out using an ANOVA followed by Dunnett's post hoc test to compare the effects of the individual pharmacological treatments with the DIV2+ group.
*p < 0.05.