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. Author manuscript; available in PMC: 2019 Aug 22.
Published in final edited form as: Clin Sci (Lond). 2019 Jul 15;133(13):1505–1521. doi: 10.1042/CS20181022

Figure 7. Incubation with EX-527 offset the negative effects of NR on cell injury in DOX-simulated cardiomyocytes.

Figure 7.

Neonatal cardiomyocytes were preincubated with NR in combination with EX-527 or vehicle for 30 min and then with DOX or saline for 24 h. Apoptosis was determined by caspase-3 activity (A) and DNA fragmentation (B). Cell viability (C) and LDH in culture media were measured (D). ROS production (E) and MDA (F) were assayed. (G and H) Western blot analysis was performed to determine phosphorylated H2A.X (p-H2A.X) and total H2A.X. (G) A representative western blots for p-H2A.X and H2A.X. (H) Quantitation of p-H2A.X/H2A.X ratio. Data are mean ± S.D., n = four different cell cultures. *P<0.05 versus vehicle, †P<0.05 versus DOX + vehicle and ‡P<0.05 versus DOX + NR.