Thiostrepton decreases the expression of antiapoptotic genes and increases the expression of proapoptotic genes. A, Heatmap showing changes in gene expression profile after thiostrepton treatment. B, qRT-PCR analysis of altered genes after thiostrepton treatment in A2780 ovarian cancer cells. A2780 cells were treated with 5 μmol/L of thiostrepton for 0, 6,8, 9, and 10 hours before total RNA extraction for RT-PCR. Data are shown as mean ± SEM in log2. Results represent experiments performed in triplicates.