Synaptic incorporation of GluA2-lacking AMPARs in CA1 pyramidal neurons following prolonged TTX treatment. A, Left, Representative traces and event amplitude scatter plots of evoked AMPAR-EPSCs before and after NASPM administration (20 μm). Right, Normalized average amplitude of evoked AMPAR-EPSCs. B, Current traces and event amplitude scatter plots of spontaneous AMPAR-EPSCs (i.e., no TTX during recording; AMPAR-sEPSC) before (baseline) and 15–20 min after onset of NASPM (20 μm) administration. C, Average AMPAR-sEPSC amplitudes before (baseline) and 15–20 min after NASPM administration. D, Current traces and event amplitude scatter plots of AMPAR-mEPSCs (i.e., recorded in TTX) before and 15–20 min after onset of NASPM administration. E, Average AMPAR-mEPSC amplitudes before (baseline) and 15–20 min after NASPM administration.