Table 2.
List of primary antibodies
| Antibody | Target | Origin | Working concentration | Reference |
|---|---|---|---|---|
| RMO270 (Invitrogen) | A phosphorylation-independent epitope of NF-M | Mouse monoclonal | 2 μg/ml (Western blot/immunofluorescence)a | Szaro et al., 1989 |
| 3C2 (Santa Cruz Biotechnology) | hnRNP K | Mouse monoclonal | 1:1000 (Western blot); 1:200 (immunofluorescence)b | Liu et al., 2008 |
| 6C5 (Ambion) | GAPDH | Mouse monoclonal | 5 μg/ml (Western blot) | |
| JDR0.3B8 (Sigma-Aldrich) | β-Tubulin I+II (neuron-specific in X. laevis) | Mouse monoclonal | 1:200 (immunofluorescence)a | Moody et al., 1996 |
| 14a9 supernatant (Developmental Studies Hybridoma Bank, University of Iowa) | X. laevis nuclear lamins II/III | Mouse monoclonal | 1:15 (immunofluorescence)c | |
| GFP (Rockland) | EGFP | Goat polyclonal | 1:1000 (Western blot) | |
| Tau | Tau-3′, targeting the C-terminal region conserved in all isoforms | Rabbit polyclonal | 1:2000 (Western blot) | Black et al., 1996; Liu and Szaro, 2011 |
| H3 (Active Motif) | Histone H3, C-terminal | Rabbit polyclonal | 1:15,000 (Western blot) | |
| pS189 | X. laevis hnRNP K, phosphorylated at serine 189 | Rabbit polyclonal | 1:50 (immunofluorescence)b | This study (Fig. 2) |
| XIF3 | X. laevis peripherin | Rabbit polyclonal | 1:500 (immunofluorescence)b | Liu et al., 2008; Liu and Szaro, 2011 |
| 81E11 (Cell Signaling Technology) | Phospho-JNK (Thr183/Tyr185) | Rabbit polyclonal | 1:500 (Western blot) | |
| Total JNK (Cell Signaling Technology) | SAPK/JNK | Rabbit polyclonal | 1:1000 (Western blot) | |
| D13.14.4E XP (Cell Signaling Technology) | Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) | Rabbit monoclonal | 1:2000 (Western blot) | |
| 137F5 (Cell Signaling Technology) | p44/42 MAPK (ERK1/2) | Rabbit polyclonal | 1:5000 (Western blot) | |
| 5G10 (Cell Signaling Technology) | S6 ribosomal protein | Rabbit monoclonal | 1:1000 |
SAPK, Stress-activated protein kinase.
aFixation in 4% paraformaldehyde in 0.1 m sodium phosphate buffer, pH 7.4.
bFixation in 100% methanol.
cFixation in dithiobis[succinimidyl propionate] (1 mg/ml; Pierce/Thermo Fisher Scientific) in phosphate buffer/40% DMSO.